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Avadis NGS September 15, 2011

Feature Highlight

Quality Control in Avadis NGS

The adoption of next-generation sequencing technologies will undoubtedly pave the way for biologists and clinical researchers to solve various mysteries in biology. However, these new technologies are undergoing continuous development as the reads they output are affected by human and machine errors during the sequencing process. As a result, scientists working with sequence data need to know how to perform quality control (QC) -- an essential process for producing reliable and reproducible results from sequence analysis.

Above: A quality control heatmap illustrating base qualities in Illumina data

Avadis NGS is one of the few bioinformatics tools featuring effective QC capabilities, in addition to a comprehensive analysis pipeline for RNA-Seq, DNA-Seq, and ChIP-Seq data, all within the same application. After importing aligned and mapped reads data into Avadis NGS, you may use the following quality inspection visualizations and read filters to yield high quality reads for analysis:
  • Alignment Score and Mapping Quality visualizations
  • Alignment Status and Base Quality visualizations for Illumina data
  • Fix Mate Status option to correct or remove inconsistent reads in paired data
  • Filters aplenty to remove unwanted and duplicate reads

To learn more about quality control of sequence data, attend this month's webinar or watch the video tutorials.

- The Avadis NGS Team

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