Grant Winner Highlight
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This month's grant winner highlight from our grant awards program is Seth Masters, Ph.D., Lab Head at the Trinity College in Dublin. The lab is focused on inflammation, specifically innate immune pathways that are triggered during infection or chronic disease to make inflammatory cytokines. They identified that miR-223 reduces production of the cytokine IL-1b. They now want to find out which inflammatory cytokines are directly targeted by miR-223 and other miRNA expressed from inflammatory cells like monocytes. They will be using the LightSwitch Luciferase Assay System and 5 putative 3’UTR GoClone reporter targets which are all cytokines produced during infection and chronic inflammatory disease. Read more.
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Featured Publication
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Antiproliferative Factor Regulates Connective Tissue Growth Factor in T24 Bladder Carcinoma
Antiproliferative factor (APF) is elevated in patients with bladder disease, interstitial cystitis (IC). APF inhibits the proliferation of bladder epithelial cells by binding to cytoskeleton associated protein 4 (CKAP4) and altering the transcription of genes involved in proliferation, cellular adhesion, and tumorigenesis. Partly by using SwitchGear Genomics Promoter GoClone reporters, the researchers uncovered specific mechanisms that control APF's antiproliferative effects. Specifically, they found that APF-treated T24 bladder carcinoma cells resulted in a 5-fold activation of the connective tissue growth factor (CCN2) proximal promoter and that siRNA-mediated knockdown CKAP4 inhibited CCN2 upregulation. Read more.
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